Oil of Jan Steen: The Doctor‘s Visit. It is seen in the seventeenth century painting a picture of a doctor examining a ribbon soaked with urine from the patient and burnt looking color to indicate positivity. An ancient Egyptian papyrus that account to detect pregnancy women had to urinate for several days on wheat and barley seeds. If after a while germinated barley, the child would be male, if germinated wheat, would be a woman, otherwise no seeds germinated, the woman was not pregnant (apparently no mention of the papyrus which was the diagnosis if germinated both). Studies done in 1963 showed that the urine of a pregnant woman promoted germination to 70 of the time, never did while that of nonpregnant women or men. Citation needed In 1928 German gynecologists Selmar Aschheim and Bernhard Zondek developed the following method. injected small amounts of urine in prepubertal female rats twice a day for 3 successive days.After about 100 hours the rats were sacrificed and inspected their ovaries. If they were enlarged, had an 80 chance that the woman was pregnant. Cinergy Health In 1930, Collip and colleagues found that pregnant women’s urine contained-then discovered they were actually pieces of it hormone-human chorionic gonadotropin, HCG, now known by the acronym of his name in English Human Chorionic Gonadotropin. In 1942, the cytologist Argentine Eduardo de Robertis, which at that time was Chief of Practical exclusive of the chair of Histology, School of Medicine, University of Buenos Aires and head of the Section of Cytology and the Institute of Anatomy Histophysiology Embryology-General and found that HCG acts on Sertoli cells resulting in the expulsion of sperm in the toads.His classmate Carlos Galli Mainini, while working at the Hospital Rivadavia in Buenos Aires between 1942 and 1947, concluded that if a pregnant woman eliminated HCG in urine injection into toads cause maturation and expulsion of their sperm. He injected urine of pregnant women in the dorsal lymph sac of male toads Bufo arenarium after 2 or 3 hours examined his urine microscopically observing the presence of sperm. The method, later known as Galli reaction Mainini or Test of the Frog, provided an effective and economical way of early diagnosis of pregnancy, and it was used heavily in Argentina and Latin America for many years. Judging by the lack of references on the Internet, it was not broadcast in Europe and America, where apparently other biological methods were used until the development of immunological some decades later.